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fecE [2019-05-31 15:18:31]
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fecE [2019-05-31 15:18:31]

iron/citrate ABC transporter (permease)
locus
BSU07500
pI
10.06
mw
35.06 kDa
protein length
333 aa Sequence Blast
gene length
1002 bp Sequence Blast
function
iron uptake
product
iron/citrate ABC transporter (permease)
essential
no
synonyms
yfmE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    823,716 824,717

    The protein

    Protein family

  • FecCD subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|BDAA56484E05DA3C1CB0CA09873A365657999679|FecD], [protein|EC98685EE0E3CE6CE8DC33409481AE96315279AC|FhuG], [protein|34A7E22B4EF118EF5A76F8CC2DABBB023BC9A2E9|YfhA]

    • Structure

  • [PDB|4G1U] (from ''Yersinia pestis'', the [protein|BDAA56484E05DA3C1CB0CA09873A365657999679|FecD]-[protein|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|FecE]-[protein|E76640F895261DA34AD2342B54B43D11857AEA9A|FecF] complex, 37% identity) [Pubmed|23142986]
  • [PDB|4R9U], the ''E. coli'' BtuC-BtuD complex, BtuC shares 35% identity, 66% similarity with YfmE, [Pubmed|25402482]

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|569510093E5B69A0368205920A21A81CADF030E9|fecC]-[gene|BDAA56484E05DA3C1CB0CA09873A365657999679|fecD]-[gene|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]-[gene|E76640F895261DA34AD2342B54B43D11857AEA9A|fecF]
    description
    [Pubmed|16672620]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [Pubmed|16672620,12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,16672620,12354229]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C245 (yfmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2243 NBRP B. subtilis, Japan]
  • BKE07500 ([gene|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTGTTTTCTGGTTGTTT, downstream forward: _UP4_AGGAAACAGTAGGGAGAGGA
  • BKK07500 ([gene|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTGTTTTCTGGTTGTTT, downstream forward: _UP4_AGGAAACAGTAGGGAGAGGA

    • References

  • 10092453,16672620,25402482,23142986,29133393